Reporter

Part:BBa_K3544005:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)


yeGFP: a green fluorescent protein in S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

2020 SCU-China use this protein as a reporter in the yeast. The fluorescence of yeGFP can be detected easily and can be distinguished with DsRed. For more information, please view https://2020.igem.org/Team:SCU-China/Design.

Source

Aequorea victoria

References

Chen J, Zhou J, Bae W, Sanders CK, Nolan JP, Cai H. A yEGFP-based reporter system for high-throughput yeast two-hybrid assay by flow cytometry. Cytometry A. 2008 Apr;73(4):312-20. doi: 10.1002/cyto.a.20525. PMID: 18307271.